Computational approaches to facilitate epitope-based HLA matching in solid organ transplantation
K. Geneugelijk, J. Wissing, D. Koppenaal, M. Niemann, E. Spierings
Chair(s): Dr. Junior M. Lardy
Thursday 9 march 2017
13:00 - 13:15h
Categories: Poster - Basaal
Parallel session: Postersessie XV - Basaal 3
Epitope-based HLA matching has been emerged over the last few years as an improved method for HLA matching in solid organ transplantation. The epitope-based matching concept has been incorporated both in the PIRCHE-II and the HLAmatchmaker algorithm to find the most suitable donor for a recipient. For these algorithms, high-resolution HLA typing of both donor and recipient is required. Since high-resolution typing is often not available, we developed a computational method which allows epitope-based HLA matching from serological split level HLA typing relying on HLA haplotype frequencies. To validate this method, we simulated a donor-recipient population for which PIRCHE-II and eplet values were calculated when using both high-resolution HLA typing and serological split level HLA typing. The majority of the serological split level HLA-determined ln(PIRCHE-II)/ln(eplet) values do not or only slightly deviate from the reference group of high-resolution HLA-determined ln(PIRCHE-II)/ln(eplet) values. This deviation was slightly increased when HLA-C or HLA-DQ was omitted from the input and was substantially decreased when using two-field resolution HLA typing of the recipient and serological split level HLA typing of the donor. Thus, our data suggest that our computational approach is a powerful tool to estimate PIRCHE-II/eplet values when high-resolution HLA typing is not available.
